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Inventors

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Projects Sharing Researchers

Researchers

  • Heather Desaire
  • Jennifer Laurence
  • Melinda Toumi
  • Jamie Wenke

Communicate

Details

Project TitleNovel Platform of Protein Production, Amplification, and Control
Track Code10KU008L
Websitehttp://kuic.dept.ku.edu/sites/default/files/Novel_Platform_of_Protein_Production_Amplification_and_Control_Technology_Profile.pdf
Short Description

The core invention surrounds a novel glycan tagging system, which facilitates protein secretion, purification, and increased yields of production.

Abstract
 
Tagsprotein production, glycan tagging
 
Posted DateSep 3, 2013 12:25 PM

Researcher

Name
Heather Desaire
Jennifer Laurence
Melinda Toumi
Jamie Wenke

Manager

Name
Matthew Koenig

Benefits

  • Increased yield and purity of folded functional protein 
  • Dramatically reduced protein production costs compared to existing systems
  • Quick and easy protein insertion into delivery expression system 
  • Applicability to production of all proteins
  • Adaptability to other cell lines, such as a yeast expression systems and/or insect cells

Applications

The technology is broadly applicable to many areas of biomedical research from manufacturing and production to drug discovery.

Overview

In nature, N-linked glycans attached to proteins signal the proteins for secretion. Lectin affinity is an established and effective method to isolate naturally glycosylated proteins from cellular expression systems. 

This platform leverages these principles for the production of proteins by using a glycan tag as a secretion signal and purification handle in a mammalian cell line. This technology is transformative because it provides higher protein yield and lower costs of production than existing platforms overcoming many of the problems of low yield, refolding, and activity control.

Why it is better

Recent advances to the core platform allow for significant time savings, greater control of protein product production, and the achievement of high yield expression of proteases, glycosidases, and kinases, which are notoriously hard to produce in high yields due to cross reactions caused by their high native activity levels.

Intellectual Property

Patent Number Issue Date Type Country of Filing
WO/2011/059828 None Utility United States


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